Journal: STAR Protocols

Article Title: Protocol for acute oxidative stress induction in third-instar Drosophila melanogaster larvae

doi: 10.1016/j.xpro.2026.104466

Figure Lengend Snippet: Preparation of materials for oxidative stress treatment (A) Materials required for oxidative stress treatment setup, including Eppendorf tubes, colorless transparent Saran wrap, and laboratory tape. (B) Add approximately 1.5 mL of liquid agarose gel to each Eppendorf tube. After solidification, ensure the upper surface of the gel is positioned approximately 1.5 mm below the tube opening. (C) Transfer larvae onto the solidified agarose medium in each tube. Add PBS to maintain humidity and seal the tube opening with Saran wrap. (D) Create ventilation holes in the Saran wrap with fine-pointed forceps. Blue circles indicate the recommended hole size and distribution pattern.

Article Snippet: Fine-pointed stainless-steel forceps , Ideal-tek , Cat# 5.SA.

Techniques: Agarose Gel Electrophoresis